2QLV
Crystal structure of the heterotrimer core of the S. cerevisiae AMPK homolog SNF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.330, 81.840, 174.740 |
| Unit cell angles | 90.00, 102.22, 90.00 |
Refinement procedure
| Resolution | 29.540 - 2.600 |
| Rwork | 0.239 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.071 | 0.385 |
| Number of reflections | 44415 | |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 93.3 | 71.3 |
| Redundancy | 2.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 250 mM ammonium citrate, 15% PEG3350, 1mM AMP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
