2QLR
Crystal structure of human kynurenine aminotransferase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-06-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.505, 70.975, 121.133 |
| Unit cell angles | 90.00, 101.10, 90.00 |
Refinement procedure
| Resolution | 30.110 - 2.300 |
| R-factor | 0.24295 |
| Rwork | 0.242 |
| R-free | 0.25601 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x0m |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.982 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.110 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.119 | 0.422 |
| Number of reflections | 96642 | |
| Completeness [%] | 92.2 | 75.8 |
| Redundancy | 3.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM HEPES, 15% PEG 10000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
