2QLL
Human liver glycogen phosphorylase- GL complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 123.986, 123.986, 125.695 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.500 - 2.560 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.27600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 2.710 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.075 | 0.471 |
| Number of reflections | 37156 | |
| <I/σ(I)> | 12.47 | 3.1 |
| Completeness [%] | 98.6 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 8 mg/ml protein, 0.1 M TRIS, 8% PEG 8000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
