2QLG

mPlum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Collection date	2005-09-25
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	61.192, 78.276, 96.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.173
Rwork	0.173
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g7k
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.800
Number of reflections	43227

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		200mM NaCl, 100mM Tris pH8.5, 30% PEG 3400