2QLE
GFP/S205V mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Collection date | 2006-06-18 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.832, 151.496, 59.729 |
| Unit cell angles | 90.00, 110.23, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.590 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| Data scaling software | HKL-2000 |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.590 |
| Number of reflections | 119117 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 298 | 100mM Imidazol pH 8.0, 1.1M Na Citrate at room temperature for 6-7 months., temperature 298K |
