2QKU

The 5th PDZ Domain of InaD in 10mM DTT

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-18
Detector	ADSC QUANTUM 315
Wavelength(s)	.97965
Spacegroup name	P 43 2 2
Unit cell lengths	67.700, 67.700, 162.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.770 - 2.200
R-factor	0.206
Rwork	0.206
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.410
High resolution limit [Å]	2.200	2.200
Number of reflections	35921
<I/σ(I)>	10.4	2.1
Completeness [%]	97.9	96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.3M Na Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.3M Na Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.3M Na Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.3M Na Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K