2QKT

Crystal Structure of the 5th PDZ domain of InaD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-08-30
Detector	MAC Science DIP-2030
Wavelength(s)	1.54
Spacegroup name	P 41 21 2
Unit cell lengths	95.740, 95.740, 48.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.050
Rwork	0.224
R-free	0.24200
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.160
High resolution limit [Å]	2.050	2.050
Number of reflections	14738
<I/σ(I)>	0.443	16.7
Completeness [%]	99.7	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.3M Sodium Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.3M Sodium Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.3M Sodium Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.3M Sodium Citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K