2QKR
Cryptosporidium parvum cyclin-dependent kinase cgd5_2510 with indirubin 3'-monoxime bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-04-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.133, 64.133, 201.153 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.670 - 2.600 |
| R-factor | 0.2494 |
| Rwork | 0.247 |
| R-free | 0.29960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ob3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.243 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.049 | 0.837 |
| Number of reflections | 17311 | |
| <I/σ(I)> | 18.2 | 2.9 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 8% PEG 20000, 16% Glycerol, 0.1 M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
