2QKP
Crystal structure of C-terminal domain of SMU_1151c from Streptococcus mutans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.999, 53.911, 79.924 |
| Unit cell angles | 90.00, 106.05, 90.00 |
Refinement procedure
| Resolution | 29.400 - 1.750 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24100 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.347 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.059 | 0.395 |
| Number of reflections | 63330 | |
| <I/σ(I)> | 34 | 3.28 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Hepes pH 7.5, 20% PEG 3000, 0.2M Sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
