2QKD
Crystal structure of tandem ZPR1 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978715 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 55.656, 142.597, 67.219 |
| Unit cell angles | 90.00, 114.05, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.20835 |
| Rwork | 0.206 |
| R-free | 0.24736 |
| Structure solution method | SAD |
| Starting model (for MR) | heavy atom model |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.265 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.348 |
| Number of reflections | 62931 | |
| <I/σ(I)> | 29 | 3.16 |
| Completeness [%] | 98.8 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 14% PEG 6000, 0.2M ammonium sulfate,10% glycerol,2mM tris(2-carboxyethyl)phosphine,0.1M tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
