2QJZ

Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9798, 1.1271, 0.7999
Spacegroup name	P 1 21 1
Unit cell lengths	29.334, 65.220, 63.091
Unit cell angles	90.00, 96.24, 90.00

Refinement procedure

Resolution	45.220 - 1.250
R-factor	0.177
Rwork	0.174
R-free	0.19800
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.033
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.290
High resolution limit [Å]	1.250	1.250
Rmerge	0.054	0.321
Number of reflections	63420
<I/σ(I)>	13.8	3.2
Completeness [%]	97.4	95.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	11.5% 2-propanol, 190 mM Sodium citrate, 100 mM Hepes, Protein concentration 20 mg/ml, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7	293	11.5% 2-propanol, 190 mM Sodium citrate, 100 mM Hepes, Protein concentration 20 mg/ml, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K