2QJY
Crystal structure of rhodobacter sphaeroides double mutant with stigmatellin and UQ2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.75 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 351.891, 147.042, 161.312 |
| Unit cell angles | 90.00, 104.25, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.400 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fyn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.680 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.107 | 0.577 |
| Number of reflections | 293559 | |
| <I/σ(I)> | 14.32 | 1.25 |
| Completeness [%] | 94.0 | 74.7 |
| Redundancy | 5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 288 | 50 mM BisTris 7.2pH, 0.5% beta-octylglucoside, 0.1% sucrose mono caprate, 7% PEG400, 5-10 mM Sr nitrate, 105 Glycerol, 200 mM NaCl, 5 mM NaN3, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
