2QJP
Crystal structure of wild type rhodobacter sphaeroides with stigmatellin and antimycin inhibited
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.75 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.061, 146.520, 141.003 |
| Unit cell angles | 90.00, 110.21, 90.00 |
Refinement procedure
| Resolution | 17.980 - 2.600 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fyn |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.710 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 0.670 |
| Number of reflections | 156392 | |
| <I/σ(I)> | 10.51 | 1.42 |
| Completeness [%] | 98.8 | 94.3 |
| Redundancy | 4.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 288 | 50 mM Tris, 0.5% b-OG, 0.12% Sucrosemonocaprate, 6.39% PEG400, 1.50% PEG3350, 10 mM Strontium nitrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
