2QJO
crystal structure of a bifunctional NMN adenylyltransferase/ADP ribose pyrophosphatase (NadM) complexed with ADPRP and NAD from Synechocystis sp.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2006-04-14 |
| Wavelength(s) | 0.97874, 0.97891, 0.984 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 201.540, 201.540, 98.695 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.480 - 2.600 |
| R-factor | 0.19568 |
| Rwork | 0.194 |
| R-free | 0.22774 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.571 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 70016 |
| Completeness [%] | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM Tris, 1.5 M Li2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
