2QJK
Crystal Structure Analysis of mutant rhodobacter sphaeroides bc1 with stigmatellin and antimycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 352.291, 147.396, 160.762 |
| Unit cell angles | 90.00, 104.13, 90.00 |
Refinement procedure
| Resolution | 18.000 - 3.100 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fyn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.168 | 0.849 |
| Number of reflections | 143931 | |
| <I/σ(I)> | 9.81 | 1.07 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.35 | 288 | 7.5% PEG 400, 0.2M NaCl, 0.2M Histidine, 0.1M Bistris, 10 mM Sr(NO3)2, 0.5% betaoctyl glucopyranoside, 0.02% cymal-6, 10% Glycerol, 5 mM NaN3, 1 mM stigmatellin, 1 mM Antimycin , pH 7.35, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
