2QJD
Crystal Structure of Novel Immune-Type Receptor 10 Extracellular Fragment Mutant N30D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.952, 94.952, 67.668 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.240 - 2.440 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.460 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.071 | 0.368 |
| Number of reflections | 13266 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 98.7 | 80.6 |
| Redundancy | 4.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 24% PEG 4000, 0.08M Tris HCL, 0.16M Magnesium Chloride, 20% Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
