2QJ8
Crystal structure of an aspartoacylase family protein (mlr6093) from mesorhizobium loti maff303099 at 2.00 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978662 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.054, 86.140, 94.632 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.643 - 2.000 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.786 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.643 | 29.643 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.105 | 0.044 | 0.782 |
| Total number of observations | 2158 | 12285 | |
| Number of reflections | 45402 | ||
| <I/σ(I)> | 11.5 | 13.4 | 1.7 |
| Completeness [%] | 99.8 | 95.9 | 98.7 |
| Redundancy | 4.6 | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | NANODROP, 20.0% PEG 3350, 0.2M Potassium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
