2QJ7
PYP ultra-high resolution of a bacterial photoreceptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.85 |
| Spacegroup name | P 65 |
| Unit cell lengths | 40.621, 40.621, 117.734 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.200 - 1.050 |
| R-factor | 0.16889 |
| Rwork | 0.168 |
| R-free | 0.18257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nwz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.397 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.239 | |
| Number of reflections | 26677 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 90.6 | 97.7 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 39% PEG 3000, 50 mM Tris pH 9, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
