2QIZ

Structure of the yeast U-box-containing ubiquitin ligase Ufd2p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-21
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97945
Spacegroup name	P 21 21 21
Unit cell lengths	65.502, 122.989, 176.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.560
R-factor	0.226
Rwork	0.221
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 2QJ0
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.560	2.560
Number of reflections	46444
<I/σ(I)>	21.6	2.1
Completeness [%]	99.2	95.2
Redundancy	4.2	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	293	20% PEG 3350, 0.2M tri-potassium citrate, 5mM DTT, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K