2QIY
yeast Deubiquitinase Ubp3 and Bre5 cofactor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9176 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.100, 90.240, 101.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.450 - 1.690 |
| R-factor | 0.2084 |
| Rwork | 0.207 |
| R-free | 0.22458 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1ZX2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.469 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.450 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.371 | |
| Number of reflections | 50599 | |
| <I/σ(I)> | 3.2 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 9 | 293 | 10%(w/v) PEG 20000, 100mM Bicine, 2% Dioxan, pH 9.0, EVAPORATION, temperature 293K |
