2QIK
Crystal structure of YkqA from Bacillus subtilis. Northeast Structural Genomics Target SR631
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.577, 62.131, 55.527 |
| Unit cell angles | 90.00, 101.69, 90.00 |
Refinement procedure
| Resolution | 19.350 - 1.350 |
| R-factor | 0.133 |
| Rwork | 0.131 |
| R-free | 0.17600 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.056 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.08) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
| Rmerge | 0.041 | 0.028 | 0.179 |
| Number of reflections | 55141 | ||
| <I/σ(I)> | 18.8 | ||
| Completeness [%] | 99.0 | 90.1 | 99.7 |
| Redundancy | 3.8 | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 277 | 0.1 M NaCl, 0.1 Na Citrate, 60% PEG 400, MICROBATCH UNDER OIL, temperature 277K |
