2QI2
Crystal structure of the Thermoplasma acidophilum Pelota protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 98.064, 98.064, 150.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 2.900 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26300 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.040 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.095 | 0.465 |
| Number of reflections | 15309 | |
| <I/σ(I)> | 11.1 | 3.4 |
| Completeness [%] | 98.5 | 90.1 |
| Redundancy | 19.1 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 297 | 100mM tri-sodium citrate (pH5.6), '1.0M mono-ammonium dihydrogen phosphate, pH7.4, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 297 | 100mM tri-sodium citrate (pH5.6), '1.0M mono-ammonium dihydrogen phosphate, pH7.4, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
