2QHL

Crystal Structure of Novel Immune-Type Receptor 10 Extracellular Fragment from Ictalurus punctatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-27
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	90.246, 90.246, 136.352
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.560
Rwork	0.196
R-free	0.22600
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.570
High resolution limit [Å]	1.560	5.330	1.560
Rmerge	0.047	0.033	0.460
Number of reflections	91403
<I/σ(I)>	12.7
Completeness [%]	99.6	94.6	95.6
Redundancy	7	6.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	1.5M Na Citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K