2QH9
The crystal structure of a protein of unknown function from Archaeoglobus fulgidus DSM 4304
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.705, 105.337, 46.707 |
| Unit cell angles | 90.00, 102.39, 90.00 |
Refinement procedure
| Resolution | 52.700 - 1.800 |
| R-factor | 0.22547 |
| Rwork | 0.224 |
| R-free | 0.25471 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.509 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 1.845 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.111 | 0.723 |
| Number of reflections | 30769 | |
| <I/σ(I)> | 22.9 | 1.8 |
| Completeness [%] | 98.4 | 94.12 |
| Redundancy | 8.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2M Ammonium acetate, 0.1M HEPES, 25% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
