2QH7

MitoNEET is a uniquely folded 2Fe-2S outer mitochondrial membrane protein stabilized by pioglitazone

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	46.806, 49.621, 59.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.000 - 1.500
R-factor	0.184
Rwork	0.182
R-free	0.22300
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.717
Data reduction software	MOSFLM
Data scaling software	XSCALE
Phasing software	SOLVE (2.12)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.000		1.550
High resolution limit [Å]	1.500	2.560	1.500
Rmerge	0.058	0.054	0.754
Number of reflections	21479
<I/σ(I)>	30.92	65.4	3.2
Completeness [%]	94.7	100	66.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1
1
1