2QH0
Crystal structure of a glyoxalase from clostridium acetobutylicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.100, 71.100, 63.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.480 - 2.450 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.29900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.060 | |
| Number of reflections | 6249 | |
| <I/σ(I)> | 17.3 | 3.1 |
| Completeness [%] | 97.2 | 97.4 |
| Redundancy | 24.8 | 20.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | HEPES Buffer, pH 7.5, Isopropanol, PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
