2QGX
Ubiquitin-conjugating enzyme E2Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 60.390, 60.390, 172.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.722 - 2.560 |
| Rwork | 0.168 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zuo |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.338 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.722 | 2.710 | |
| High resolution limit [Å] | 2.550 | 5.400 | 2.550 |
| Rmerge | 0.039 | 0.028 | 0.315 |
| Number of reflections | 22493 | ||
| <I/σ(I)> | 15.81 | 32 | 3.1 |
| Completeness [%] | 96.7 | 99.2 | 89.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 22% PEG4000, 0.1 M Tris-Cl, pH7.5, 0.12 M MgCl2, 1 mM DTT, vapor diffusion, temperature 298K |
