2QGU

Three-dimensional structure of the phospholipid-binding protein from Ralstonia solanacearum Q8XV73_RALSQ in complex with a phospholipid at the resolution 1.53 A. Northeast Structural Genomics Consortium target RsR89

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	42.495, 46.478, 117.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.950 - 1.500
R-factor	0.18667
Rwork	0.185
R-free	0.21142
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.020
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.053	0.152
Number of reflections	34787
<I/σ(I)>	25.6	7.6
Completeness [%]	92.5	84.3
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1M Tris-HCl, 25% w/v PEG6000, 0.1M Trimethylamine, 10% Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K