2QGR

Structure of the R178A mutant of delta PDZ DegS protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	123
Detector technology	IMAGE PLATE
Collection date	2007-05-27
Detector	RIGAKU RAXIS IV
Spacegroup name	H 3
Unit cell lengths	70.216, 70.216, 119.228
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.460 - 2.700
Rwork	0.249
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qf0
RMSD bond length	0.002
RMSD bond angle	12.010
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge		0.201
Number of reflections	5938
Completeness [%]	98.2	87.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	293	50 mM NaCacodylate pH 6.5, 100 mM NaCitrate, 20% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K