2QGG
X-Ray structure of the protein Q6F7I0 from Acinetobacter calcoaceticus AmMS 248. Northeast Structural Genomics Consortium target AsR73.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Detector technology | CCD |
| Collection date | 2007-04-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 44.899, 37.588, 69.200 |
| Unit cell angles | 90.00, 97.23, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.400 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.078 | 0.243 |
| Number of reflections | 9040 | |
| Completeness [%] | 97.9 | 85.7 |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% PEG 20000, 0.1M Potassium acetate, 0.1M MOPS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
