2QFZ

Crystal structure of human TBC1 domain family member 22A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-29
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1000
Spacegroup name	P 1 21 1
Unit cell lengths	42.911, 121.515, 66.278
Unit cell angles	90.00, 93.64, 90.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.202
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g77
RMSD bond length	0.017
RMSD bond angle	1.395
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.152	0.074	0.966
Number of reflections	39313	3997	3935
<I/σ(I)>	6.5
Completeness [%]	100.0
Redundancy	6.4	6.2	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	28.0% PEG400, 0.2M Calcium chloride, 0.1M Sodium HEPES, pH 7.5, VAPOR DIFFUSION, temperature 291K