2QFL
Structure of SuhB: Inositol monophosphatase and extragenic suppressor from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-11 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.767, 45.447, 71.758 |
| Unit cell angles | 90.00, 125.43, 90.00 |
Refinement procedure
| Resolution | 47.000 - 1.900 |
| R-factor | 0.21331 |
| Rwork | 0.209 |
| R-free | 0.28555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Hybrid of human and MJ IMPase |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.851 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.970 |
| High resolution limit [Å] | 1.820 | 1.900 |
| Rmerge | 0.050 | 0.270 |
| Number of reflections | 15115 | |
| <I/σ(I)> | 19.9 | 4.1 |
| Completeness [%] | 95.0 | 36 |
| Redundancy | 3.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 20% PEG 3350, 0.2M ammonium acetate, 0.05M TRIS, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
