2QFJ

Crystal Structure of First Two RRM Domains of FIR Bound to ssDNA from a Portion of FUSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 31
Unit cell lengths	63.135, 63.135, 82.589
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.590 - 2.100
R-factor	0.258
Rwork	0.258
R-free	0.29500
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.059	0.038	0.370
Number of reflections	20889
<I/σ(I)>	14.6
Completeness [%]	97.5	90.3	92.2
Redundancy	5.1	5.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5		Reservoir: 0.1 M Tris-HCl, 32% PEG 4000, pH 8.5 mixed 1:1 with protein/DNA complex in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, vapor diffusion, hanging drop
1	VAPOR DIFFUSION, HANGING DROP	8.5		Reservoir: 0.1 M Tris-HCl, 32% PEG 4000, pH 8.5 mixed 1:1 with protein/DNA complex in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, vapor diffusion, hanging drop
1	VAPOR DIFFUSION, HANGING DROP	8.5		Reservoir: 0.1 M Tris-HCl, 32% PEG 4000, pH 8.5 mixed 1:1 with protein/DNA complex in 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, vapor diffusion, hanging drop

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	20 mM Tris-HCl
2	1	1	NaCl
3	1	2	0.1 M Tris-HCl
4	1	2	PEG 4000