2QF9
Crystal structure of putative secreted protein DUF305 from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.903, 52.756, 57.273 |
| Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
| Resolution | 23.830 - 1.690 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.160 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.038 | 0.172 |
| Number of reflections | 33695 | |
| <I/σ(I)> | 44.5 | 6.8 |
| Completeness [%] | 94.2 | 60 |
| Redundancy | 5.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 1M Bis-Tris pH 5.5, 50% PEG 3350, 1M Sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
