2QF7
Crystal structure of a complete multifunctional pyruvate carboxylase from Rhizobium etli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Detector technology | CCD |
| Collection date | 2006-08-18 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 234.729, 93.258, 137.222 |
| Unit cell angles | 90.00, 107.33, 90.00 |
Refinement procedure
| Resolution | 131.310 - 2.000 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.623 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.10) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 184296 | |
| <I/σ(I)> | 20.1 | 3.1 |
| Completeness [%] | 96.7 | 86 |
| Redundancy | 4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 298 | 7.5 mg/mL PC, 2.5 mM ATP-gamma-S, 1 mM ethyl CoA, 280 mM sodium formate, 70 mM sodium acetate, 60 mM calcium chloride, 5.9% PEG 8K, pH 5.6, EVAPORATION, temperature 298K |
