2QF2
Rat cytosolic PEPCK in complex with oxaloacetic acid and GDP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.506, 119.112, 86.710 |
Unit cell angles | 90.00, 107.35, 90.00 |
Refinement procedure
Resolution | 33.330 - 1.650 |
R-factor | 0.20597 |
Rwork | 0.204 |
R-free | 0.23762 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qew |
RMSD bond length | 0.009 |
RMSD bond angle | 1.188 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.082 | 0.693 |
Number of reflections | 142474 | |
<I/σ(I)> | 9 | |
Completeness [%] | 96.6 | 80.3 |
Redundancy | 6.9 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 25% PEG 3350, 0.1M HEPES pH 7.4, 10 mM MnCl2, 10 mM GDP, VAPOR DIFFUSION, HANGING DROP, temperature 298K |