2QF1
Rat cytosolic PEPCK in complex with oxaloacetic acid.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.218, 119.340, 60.808 |
| Unit cell angles | 90.00, 108.71, 90.00 |
Refinement procedure
| Resolution | 24.480 - 1.800 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qew |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.116 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.078 | 0.046 | 0.516 |
| Number of reflections | 50217 | ||
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 89.3 | 96.4 | 46.4 |
| Redundancy | 7 | 7.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 25% PEG 3350, 10 mM MnCl2, 0.1M HEPES pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
