2QES

Crystal structure of the ribosome inactivating protein PDL4 from P. dioica leaves in complex with adenine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-08
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 21
Unit cell lengths	43.521, 58.813, 98.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.240
Rwork	0.116
R-free	0.14500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qcg
RMSD bond length	0.022
RMSD bond angle	0.036
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	IlMilione
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.240	1.240
Number of reflections	72193

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION			VAPOR DIFFUSION