2QEO
Crystal Structure of Anopheles gambiae D7R4-norepinephrine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | SBC-3 |
| Wavelength(s) | 0.978743 |
| Spacegroup name | P 43 |
| Unit cell lengths | 87.807, 87.807, 43.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.710 - 2.315 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.149 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.710 | 2.390 |
| High resolution limit [Å] | 2.315 | 2.315 |
| Rmerge | 0.082 | 0.367 |
| Number of reflections | 14718 | |
| <I/σ(I)> | 7.1 | 4.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 30 %PEG 6000, 0.1M Tris HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
