2QEH
Crystal Structure of Anopheles gambiae D7r4-serotonin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792601 |
| Spacegroup name | P 43 |
| Unit cell lengths | 63.358, 63.358, 42.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.370 - 2.102 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.23800 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.965 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.370 | 2.180 |
| High resolution limit [Å] | 2.102 | 2.102 |
| Rmerge | 0.070 | 0.409 |
| Number of reflections | 9976 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 99.5 | 98.9 |
| Redundancy | 7.9 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20 %PEG 6000, 0.1 M Tris HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
