2QEC

Crystal structure of histone acetyltransferase HPA2 and related acetyltransferase (NP_600742.1) from Corynebacterium glutamicum ATCC 13032 at 1.90 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162
Spacegroup name	P 65 2 2
Unit cell lengths	79.070, 79.070, 181.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.640 - 1.900
R-factor	0.195
Rwork	0.193
R-free	0.24200
Structure solution method	MAD, MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.609
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.640	27.640	1.970
High resolution limit [Å]	1.900	4.080	1.900
Rmerge	0.071	0.036	0.905
Number of reflections	27197
<I/σ(I)>	17.36	51.7	2
Completeness [%]	99.5	99.5	99.3
Redundancy	17.62

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	NANODROP, 8.0% PEG 4000, 0.1M Acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
2	VAPOR DIFFUSION, SITTING DROP	8.5	277	NANODROP, 0.2M Li2SO4, 40.0% PEG 400, 0.1M Tris-HCl pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K