2QEB
Crystal Structure of Anopheles Gambiae D7R4-Histamine Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979115 |
| Spacegroup name | P 43 |
| Unit cell lengths | 88.394, 88.394, 43.395 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.390 - 2.003 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.105 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.390 | 2.070 |
| High resolution limit [Å] | 2.003 | 2.003 |
| Rmerge | 0.076 | 0.447 |
| Number of reflections | 22811 | |
| <I/σ(I)> | 16.4 | 2.7 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20 % PEG 6000, 0.1 M Tris HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
