2QEA

CRYSTAL STRUCTURE OF A PUTATIVE GENERAL STRESS PROTEIN 26 (JANN_0955) FROM JANNASCHIA SP. CCS1 AT 2.46 A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97905, 0.97920, 0.91837
Spacegroup name	P 61 2 2
Unit cell lengths	73.673, 73.673, 367.524
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.604 - 2.460
R-factor	0.233
Rwork	0.231
R-free	0.27500
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.371
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.604	29.604	2.520
High resolution limit [Å]	2.460	11.000	2.460
Rmerge	0.131	0.047	0.733
Total number of observations		1648	5729
Number of reflections	22684
<I/σ(I)>	5.2	11.1	1
Completeness [%]	99.8	90.7	99.8
Redundancy	6.4	5.3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	NANODROP, 0.14M CaCl2, 30.0% Glycerol, 14.0% Isopropanol, 0.1M Acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K