2QDW
Structure of Cu(I) form of the M51A mutant of amicyanin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.210, 55.360, 27.270 |
| Unit cell angles | 90.00, 95.75, 90.00 |
Refinement procedure
| Resolution | 30.000 - 0.920 |
| R-factor | 0.122 |
| Rwork | 0.121 |
| R-free | 0.14690 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | pdb code 1AAC |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.039 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 0.940 |
| High resolution limit [Å] | 0.920 | 0.920 |
| Rmerge | 0.081 | 0.259 |
| Number of reflections | 52498 | |
| <I/σ(I)> | 12 | 4.8 |
| Completeness [%] | 92.2 | 45.3 |
| Redundancy | 7.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 293 | 2.5-3.0 M phosphate 10 mg/ml amicyanin, pH 5.5, EVAPORATION, temperature 293K |
