2QDQ
Crystal structure of the talin dimerisation domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.07225 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 98.820, 98.820, 98.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.800 - 2.200 |
R-factor | 0.257 |
Rwork | 0.257 |
R-free | 0.31500 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.092 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE (2.08) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.330 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.057 | 0.531 |
Number of reflections | 8844 | |
<I/σ(I)> | 51.71 | 9.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 39.9 | 41.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 292 | 100 mM Citrate buffer (pH 4.2), 11% (w/v) PEG 3000, 200 mM Sodium Chloride, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 292 | 100 mM Citrate buffer (pH 4.2), 11% (w/v) PEG 3000, 200 mM Sodium Chloride, VAPOR DIFFUSION, SITTING DROP, temperature 292K |