2QDQ

Crystal structure of the talin dimerisation domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.07225
Spacegroup name	P 41 3 2
Unit cell lengths	98.820, 98.820, 98.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.800 - 2.200
R-factor	0.257
Rwork	0.257
R-free	0.31500
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.092
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE (2.08)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.200	2.200
Rmerge	0.057	0.531
Number of reflections	8844
<I/σ(I)>	51.71	9.2
Completeness [%]	99.9	100
Redundancy	39.9	41.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	292	100 mM Citrate buffer (pH 4.2), 11% (w/v) PEG 3000, 200 mM Sodium Chloride, VAPOR DIFFUSION, SITTING DROP, temperature 292K
1	VAPOR DIFFUSION, SITTING DROP	4.2	292	100 mM Citrate buffer (pH 4.2), 11% (w/v) PEG 3000, 200 mM Sodium Chloride, VAPOR DIFFUSION, SITTING DROP, temperature 292K