2QDN
Crystal Structure of mouse GITRL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.74332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.923, 71.514, 70.188 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.060 - 2.090 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.372 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.060 | 50.000 | 2.160 |
| High resolution limit [Å] | 2.090 | 4.500 | 2.090 |
| Rmerge | 0.043 | 0.041 | 0.121 |
| Number of reflections | 14872 | ||
| <I/σ(I)> | 27.3 | ||
| Completeness [%] | 90.3 | 100 | 52.9 |
| Redundancy | 11.2 | 12.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 15% PEG 3350, 0.1 M Succinic acid pH 7.0, Vapor diffusion, Sitting drop, temperature 298K |
