2QDL
Crystal structure of scaffolding protein TtCheW from Thermoanaerobacter tengcongensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-17 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.214, 62.235, 55.142 |
| Unit cell angles | 90.00, 115.37, 90.00 |
Refinement procedure
| Resolution | 19.760 - 2.200 |
| R-factor | 0.19619 |
| Rwork | 0.194 |
| R-free | 0.23278 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ch4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.450 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.078 | 0.286 |
| Number of reflections | 14589 | |
| Completeness [%] | 96.0 | 93.6 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 0.1M acetate buffer (pH 5.4), 0.2M Sodium Nitrate, 10% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
