2QCP
1.0 A Structure of CusF-Ag(I) residues 10-88 from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-29 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.118, 39.351, 44.423 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.680 - 1.000 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zeq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.957 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.049 | 0.383 |
| Number of reflections | 35956 | |
| <I/σ(I)> | 97.7 | 3.1 |
| Completeness [%] | 97.7 | 95.6 |
| Redundancy | 5.52 | 5.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 100 mM sodium acetate trihydrate (pH 4.6), 2.3 M ammonium sulfate, 2 mM silver nitrate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
