2QCK

Crystal structure of flavin reductase domain protein (YP_831077.1) from Arthrobacter sp. FB24 at 1.90 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-03
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97908
Spacegroup name	P 62 2 2
Unit cell lengths	60.510, 60.510, 180.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.841 - 1.900
R-factor	0.206
Rwork	0.204
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.647
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.841	29.841	1.970
High resolution limit [Å]	1.900	4.080	1.900
Rmerge	0.089	0.028	1.032
Number of reflections	16301	2971	2974
<I/σ(I)>	13.86	54.4	1.97
Completeness [%]	99.8	99.2	99.6
Redundancy	13.73

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	277	NANODROP, 40.0% PEG 600, 0.1M CHES pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K