2QCB
T7-tagged full-length streptavidin complexed with ruthenium ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 57.651, 57.651, 183.823 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.650 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.574 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.034 | 0.256 |
| Number of reflections | 21478 | |
| <I/σ(I)> | 63.4 | 3.6 |
| Completeness [%] | 89.3 | 27.8 |
| Redundancy | 10.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | protein, 26 mg/ml in water. 4-5x molar excess of ligand. reservoir, 1.0 M sodium citrate, 0.1 M cacodylate buffer, pH 6.5 , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
